Tabbatar da Yawan Oxalic Acid a cikin 'Ya'yan itatuwa da kayan lambu ta hanyar amfani da High-Performance Liquid Chromatography (HPLC)
1. Ka'idar Gwaji da Tsarin Hanya
A matsayin sinadarin acid na halitta da aka saba samu a cikin 'ya'yan itatuwa da kayan lambu, sinadarin oxalic acid yana shafar dandano da ƙimar abinci kai tsaye. Wannan gwajin yana amfani da chromatography na ruwa mai aiki mai juyi (RP-HPLC). A ƙarƙashin yanayin yanayin motsi na acidic, oxalic acid yana rabuwa da abubuwan da ke shiga tsakani ta amfani da ginshiƙin chromatographic na C18. Ana amfani da na'urar gano ultraviolet da aka saita a 210 nm don nazarin adadi, bisa ga halayen sha na UV na ƙungiyoyin carboxyl a cikin ƙwayoyin oxalic acid.
2. Tsarin Lanƙwasa na Daidaitacce da Shiri na Samfura
Ana amfani da hanyar dilution mai sauƙi don shirya mafita na yau da kullun: auna 25.0 mg na oxalic acid dihydrate daidai sannan a narkar da shi zuwa 25 mL da ruwa mai tsarki don samun maganin 1 mg/mL. Ana narkar da wannan a jere a cikin jerin mafita na yau da kullun a 50, 100, 200, 400, da 800 µg/mL, tare da kowane yawan da aka yi a cikin allura sau uku.
Don shirya samfurin, ana amfani da cirewa ta hanyar amfani da microwave: auna gram 5.00 na 'ya'yan itace/kayan lambu, ƙara 10 ml na maganin hydrochloric acid na 0.1 mol/L, a jika a zafin 60°C da microwave na tsawon mintuna 10, a tace ta hanyar matatar membrane mai girman 0.45 µm, sannan a tattara tacewar don gwaji.
3. Inganta Yanayin Chromatographic
Matakin wayar hannu shine tsarin 0.01 mol/L potassium dihydrogen phosphate buffer (pH 2.5)–acetonitrile (95:5), tare da saurin kwarara na 0.8 mL/min da zafin jiki na ginshiƙi na 35°C. Daidaita rabon acetonitrile yana nuna cewa lokacin da yanayin halitta ya wuce 10%, lokacin riƙewa na oxalic acid yana raguwa zuwa ƙasa da mintuna 3, amma kololuwar wutsiya yana faruwa. A ƙarƙashin yanayin da aka inganta na ƙarshe, lokacin riƙewa na oxalic acid shine mintuna 4.2, yana cimma cikakken rabuwa daga citric acid da malic acid da ke kusa (ƙuduri > 1.5).
4. Tabbatar da Hanyar
Tabbatar da kewayon layi yana nuna kyakkyawar alaƙar layi tsakanin yankin kololuwa da yawan haɗuwa a cikin kewayon 10-1000 µg/mL (R² = 0.9993). Iyakar ganowa (LOD), wanda aka ƙaddara ta hanyar hanyar rabon sigina zuwa hayaniya, shine 0.5 µg/mL. A cikin gwaje-gwajen maimaitawa, RSD don tantancewa guda shida na samfurin alayyafo iri ɗaya shine 1.8%. Gwaje-gwajen murmurewa na Spike a matakai uku (80%, 100%, da 120%) suna samar da matsakaicin murmurewa na 98.2%, 102.4%, da 97.8%, bi da bi, suna cika buƙatun nazarin adadi.
5. Binciken Samfuran Gaske
An gwada 'ya'yan itatuwa da kayan lambu guda shida da ake samu a kasuwa: alayyafo (356 ± 12 mg/100 g), seleri (215 ± 9 mg/100 g), tumatir (18 ± 2 mg/100 g), apple (6 ± 1 mg/100 g), ayaba (ba a gano ba), da broccoli (89 ± 5 mg/100 g). Kwatanta da hanyar ƙasa ta yau da kullun (GB 5009.277-2016) ya nuna cewa bambancin da ke tsakanin hanyoyin biyu bai kai kashi 5% ba, wanda hakan ke tabbatar da ingancin wannan hanyar. Musamman ma, hanyar HPLC tana nuna mafi girman hankali fiye da hanyoyin titration na gargajiya lokacin nazarin samfuran da ba su da abun ciki.
6. Muhimman Bayanan kula
A kula da ƙimar pH sosai yayin gwajin samfurin kafin a fara amfani da shi. Idan pH na ruwan ya wuce 3, ruwan calcium oxalate zai haifar da ƙarancin sakamakon gwaji. Ya kamata a shirya matakin da ke motsi sabo kowace rana don guje wa ruwan gishiri da toshewar ginshiƙi. Bayan kowace allura 20, a wanke ginshiƙin da acetonitrile-ruwa (30:70) na tsawon mintuna 30 don hana lalacewar ingancin ginshiƙi. Ga samfuran da ke ɗauke da launin (misali, kabeji mai launin shunayya), ana ba da shawarar a ƙara matakin tsarkakewa mai ƙarfi kafin a yi allura.
7. Umarnin Amfani da Faɗaɗawa
Ana iya faɗaɗa wannan hanyar zuwa ga gano sinadarin oxalic a cikin samfuran halittu kamar fitsari da jini. Ta hanyar daidaita tsarin aikin motsi (misali, ƙara tetrabutylammonium hydroxide), ana iya tantance sinadarin oxalic da metabolites ɗinsa a lokaci guda. Idan aka haɗa shi da na'urar gano mass spectrometry, za a iya kafa wata hanyar gano alamun da ta fi dacewa. A cikin masana'antar sarrafa abinci, wannan hanyar za ta iya samar da mahimman bayanai don zaɓar nau'ikan acid mai ƙarancin oxalic da kuma inganta hanyoyin girki.
Kamfanin Zibo Anhao Chemical Co., Ltd. – Oxalic Acid
Kamfanin Zibo Anhao Chemical Co., Ltd. amintaccen mai samar da kayayyaki ne kuma mai fitar da sinadarin oxalic acid mai inganci (matsayin masana'antu, minti 99.6) daga Shandong, China. Acid ɗinmu na oxalic acid fari ne mai ƙarfi, wanda aka fi sani da ethanedioic acid, tare da dabarar kwayoyin halitta ta C₂H₂O₄. Yana da tsarki mai yawa, ƙarancin ƙazanta, da kuma inganci mai karko a cikin rukuni-rukuni, yana biyan buƙatun yadi, fata, ƙarfe, sarrafa ƙasa mai wuya, tsaftace sinadarai, da masana'antar gini.
Muna samar da sinadarin oxalic acid mai ruwa da kuma wanda ba shi da ruwa, wanda ake samu a cikin jakunkuna masu nauyin kilogiram 25, jakunkuna masu nauyin kilogiram 50, da kuma jakunkuna masu nauyin kilogiram 1000. Tare da tsauraran hanyoyin QC, cikakkun rahotannin gwaji, da kuma tsare-tsaren samar da kayayyaki masu sassauƙa, muna tabbatar da isar da kayayyaki cikin kwanciyar hankali da kuma aiki mai dorewa ga masu siye a duniya. An fitar da kayayyakinmu zuwa kudu maso gabashin Asiya, Turai, Afirka, da Amurka, inda aka amince da inganci mai inganci da farashi mai kyau.
Zaɓi Zibo Anhao Chemical don wadatar oxalic acid mai inganci da kuma sabis na sinadarai na ƙwararru ɗaya.
Zaɓi Zibo Anhao Chemical don sinadarin oxalic acid mai tsarki wanda ke samar da aiki mai ɗorewa da ƙima ga kasuwancin ku. Tuntuɓe mu don yin oda mai yawa ko takamaiman bayanai na fasaha: info@anhaochemical.com
